Designing a soluble full-length HIV-1 gp41 trimer
AssigneeThe Catholic University of America
InventorsVenigalla B. Rao, Guofen Gao
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Abstract
Described herein is a soluble HIV-1 retrovirus transmembrane glycoprotein gp41 trimer (Soc-gp41M-Fd) containing a partial ectodomain and the cytoplasmic domain, that is fused to the small outer capsid (Soc) protein of bacteriophage T4 and the Foldon domain of the bacteriophage T4 fibritin (Fd). The gp41 trimer that has a prehairpin structure could be utilized to understand the mechanism of viral entry and as a candidate for development of HIV-1 vaccines, diagnostics and therapeutics. Other secondary embodiments of the gp41 proteins containing different modifications are also disclosed. According to one embodiment, the gp41 trimer is further attached to a cell penetration peptide (CPP). Methods of producing gp41 trimers are also disclosed.
Claims5
A method for producing a soluble HIV-1 gp41 recombinant protein comprising:
- isolating an HIV-1 gp41 recombinant protein by affinity chromatography from a supernatant of cell lysate of a cell expressing the HIV-1 gp41 recombinant protein, thereby producing purified soluble HIV-1 gp41 recombinant protein;
- wherein the HIV-1 gp41 recombinant protein comprises an amino acid sequence set forth in SEQ ID NO:
- 6, and
- wherein the purified soluble HIV-1 gp41 recombinant protein comprises soluble oligomeric HIV-1 gp41 recombinant protein and soluble trimeric HIV-1 gp41 recombinant protein.
A method for producing a soluble HIV-1 gp41 recombinant protein comprising:
- isolating an HIV-1 gp41 recombinant protein from an inclusion body fraction of a cell lysate of a cell expressing the HIV-1 gp41 recombinant protein by urea denaturation and affinity chromatography, thereby obtaining isolated soluble HIV-1 gp41 recombinant protein;
- and
- refolding the isolated soluble HIV-1 gp41 recombinant protein by slow dialysis against solutions containing incrementally decreased urea concentration, thereby producing refolded soluble HIV-1 gp41 recombinant protein;
- wherein the HIV-1 gp41 recombinant protein comprises an amino acid sequence set forth in SEQ ID NO:
- 6, and
- wherein the refolded soluble HIV-1 gp41 recombinant protein comprises soluble oligomeric HIV-1 gp41 recombinant protein and soluble trimeric HIV-1 gp41 recombinant protein.
A method for expressing a soluble HIV-1 gp41 recombinant protein comprising inducing an expression of an HIV-1 gp41 recombinant protein comprising SEQ ID NO:
- 6 in a cell, wherein the cell contains an expression vector comprising a DNA fragment encoding the HIV-1 gp41 recombinant protein comprising SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.