Polysaccharide and uses thereof
AssigneeGlycovaxyn AG
InventorsMichael T. Kowarik, Michael L. Wetter, Stefan J. Kemmler, Micha A. Häuptle, Veronica Gambillara, Manuela Mally
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Abstract
Provided herein is a novel E. coli O polysaccharide, O25B. Also provided herein are prokaryotic host cells comprising enzymes (e.g., glycosyltransferases) used in O25B production. The host cells provided herein produce O25B bioconjugates, wherein said bioconjugates comprise O25B linked to a carrier protein. Further provided herein are compositions, e.g., pharmaceutical compositions, comprising O25B and/or bioconjugates comprising O25B. Such compositions can be used as vaccines against infection with ExPEC, and may further comprise one or more additional bioconjugates.
Claims20
A composition comprising a bioconjugate of an E. coli O25B antigen covalently coupled to a carrier protein, (ii) a bioconjugate of an E. coli O1A antigen covalently coupled to a carrier protein, (iii) a bioconjugate of an E. coli O2 antigen covalently coupled to a carrier protein, and (iv) a bioconjugate of an O6 antigen covalently coupled to a carrier protein,
- wherein the E. coli O25B antigen comprises the structure of Formula O25B′:
- wherein n is an integer between 1 and 30.
A composition comprising a bioconjugate of an E. coli O25B antigen covalently bound to an Asn residue of detoxified Exotoxin A of P. aeruginosa (EPA), (ii) a bioconjugate of an E. coli O1A antigen covalently bound to an Asn residue of detoxified EPA, (iii) a bioconjugate of an E. coli O2 antigen covalently bound to an Asn residue of detoxified EPA, and (iv) a bioconjugate of an O6 antigen covalently bound to an Asn residue of detoxified EPA,
- wherein the E. coli O25B antigen comprises the structure of Formula O25B′:
- wherein n is an integer between 1 and 30.
An immunogenic composition comprising (i) a bioconjugate of an E. coli O25B antigen covalently bound to an Asn residue of detoxified Exotoxin A of P. aeruginosa (EPA), (ii) a bioconjugate of an E. coli O1A antigen covalently bound to an Asn residue of detoxified EPA, (iii) a bioconjugate of an E. coli O2 antigen covalently bound to an Asn residue of detoxified EPA, and (iv) a bioconjugate of an O6 antigen covalently bound to an Asn residue of detoxified EPA,
- wherein the E. coli O25B antigen, O1A antigen, O6 antigen, and O2 antigen comprise the following formulas, respectively:
- wherein n is an integer between 1 and 30.
Dependencies are inferred from the claim text.