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US 9,700,611 B2Granted
Live attenuated catfish vaccine and method of making
AssigneeMississippi State University
InventorsMark L. Lawrence, Attila Karsi
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AssigneeMississippi State University
InventorsMark L. Lawrence, Attila Karsi
Estimating the term…
Live attenuated bacteria vaccines against enteric septicemia of fish, especially catfish, and methods related to the same. Mutant strains of the bacteria Edwardsiella ictaluri (a pathogenic bacterial strain of Enterobacteriaceae) are provided. The mutant Edwardsiella ictaluri bacteria (or other pathogenic bacterial strain of Enterobacteriaceae) contain one or more gene deletions or disruptions that result in less virulent bacterial strains as live attenuated vaccine compositions against virulent wild-type Edwardsiella ictaluri bacteria (or other pathogenic bacterial strain of Enterobacteriaceae). The mutant strains showing the best immunological protection and safety as a vaccine are the triple mutants ESC-NDKL1 (ΔgcvPΔsdhCΔfrdA) strain and ESC-NDKL2 (ΔgcvPΔsdhCΔmdh) strain, with the ESC-NDKL1 strain providing the greatest safety and efficacy of these two triple mutants.
A composition for providing immunological protection from an enteric septicemia caused by Edwardsiella ictaluri, said composition comprises a live attenuated strain of Edwardsiella ictaluri comprising gene disrupting mutations in genes coding for three proteins in the group consisting of glycine cleavage system (gcvP), succinate dehydrogenase (sdhC), malate dehydrogenase (mdh), and fumarate reductase (frdA).
A mutant bacterial strain of Edwardsiella ictaluri comprising gene disrupting mutations in genes coding for three proteins in the group consisting of glycine cleavage system (gcvP), succinate dehydrogenase (sdhC), malate dehydrogenase (mdh), and fumarate reductase (frdA).
A composition for providing immunological protection from an enteric disease caused by a pathogenic bacterial strain of Enterobacteriaceae comprising a live attenuated strain of the pathogenic bacterial strain of Enterobacteriaceae comprising gene disrupting mutations in genes coding for three proteins in the group consisting of glycine cleavage system (gcvP), succinate dehydrogenase (sdhC), malate dehydrogenase (mdh), and fumarate reductase (frdA).
A method of providing immunological protection to an animal from an enteric disease caused by a pathogenic bacterial strain of Enterobacteriaceae in the animal comprising providing to the animal an effective amount of a live attenuated strain of the pathogenic bacterial strain of Enterobacteriaceae comprising gene disrupting mutations in genes coding for three proteins in the group consisting of glycine cleavage system (gcvP), succinate dehydrogenase (sdhC), malate dehydrogenase (mdh), and fumarate reductase (frdA).
A method of providing immunological protection to an animal from an enteric disease caused by a pathogenic bacterial strain of Edwardsiella ictaluri in the animal comprising providing to the animal an effective amount of a live attenuated strain of the pathogenic bacterial strain of Edwardsiella ictaluri comprising gene disrupting mutations in genes coding for three proteins in the group consisting of glycine cleavage system (gcvP), succinate dehydrogenase (sdhC), malate dehydrogenase (mdh), and fumarate reductase (frdA).
Dependencies are inferred from the claim text.