Regenerated tissue comprising a stratified structure of epithelial cells
AssigneeShigekazu Nakatsugawa
InventorShigekazu Nakatsugawa
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Abstract
A normal regenerated tissue is formed by exposing to radiation a connective tissue or a supporting tissue originating in an organ to thereby form a feeder layer and then transplanting epithelial cells thereon to form a stratified structure. By conveniently and surely providing regenerated tissue by the 3-dimensional culture with the use of a human-origin normal tissue as a base, it is possible to construct systems for assessing effects and side effects of chemicals such as drugs or assessing sensitivities thereof with the use of regenerated tissues as models of corresponding tissues respectively.
Claims7
An isolated regenerated tissue comprising a stratified structure of epithelial cells on an irradiated feeder layer of fibroblast and vascular endothelial cells obtained from an organ, wherein the fibroblasts, the endothelial cells and the epithelial cells are orthotopic with regard to the originating organ, wherein the organ is not skin;
- wherein the feeder layer is formed by irradiating the combination of the vascular endothelial cells and the fibroblast cells with 20 Gy or less X-ray or γ-ray;
- and wherein a ratio of the fibroblasts to the vascular endothelial cells is 10:1.
Dependencies are inferred from the claim text.