Targeted sequencing of biomolecules by pulling through a liquid-liquid interface with an atomic force microscope

A method of sequencing a polymer, comprising:

• vertically traversing the polymer from a first medium to a second medium in a chamber, wherein a first end of the polymer is connected to an atomic force microscope and a second end of the polymer is connected to a bottom surface of the chamber;
• wherein the polymer comprises blocks;
• wherein the first medium is water and the second medium is oil;
• as the polymer vertically traverses from the first medium to the second medium, measuring different measured forces corresponding to each of the blocks;
• and
• distinguishing the blocks from one another according to the different measured forces measured for each of the blocks, the distinguishing comprises:
• determining a plateau I and plateau II from the different measured forces, the plateau I defined to include a force for unwinding and stretching the polymer while in the first medium, the plateau II defined as crossing a boundary from the first medium to the second medium;
• determining a solvophobic force of one block of the polymer by a difference in the plateau I and the plateau II.