Gene encoding enzyme that reduces position 24 of steroid skeleton and plant in which expression level of the gene is lowered
AssigneeKirin Holdings Kabushiki Kaisha
InventorsNaoyuki Umemoto, Masako Otsuka
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Abstract
This invention provides a plant belonging to the family Solanaceae that does not produce cholesterols, including glycoalkaloids. This invention concerns a protein having activity of an enzyme that reduces position 24 of the steroid skeleton of a plant belonging to the family Solanaceae, a novel plant in which a gene encoding such protein is suppressed, and a method for producing and testing such plant.
Claims8
A method for producing a plant belonging to the family Solanaceae which has no traits of dwarfism and has a reduced risk for accumulation of glycoalkaloids, comprising suppressing the expression of a gene encoding an enzyme that reduces a double bond between C24 and C25 of the steroid skeleton by artificial procedure, the gene consisting of any one of DNAs (a) to (c) below:
- (a) DNA consisting of the nucleotide sequence as shown in SEQ ID NO:
- 2, 4 or 22;
- (b) DNA consisting of a nucleotide sequence having 95% or higher sequence identity with the nucleotide sequence as shown in SEQ ID NO:
- 2, 4 or 22 and encoding a protein that reduces a double bond between C24 and C25 of the steroid skeleton;
- and
- (c) DNA encoding a protein consisting of the amino acid sequence as shown in SEQ ID NO:1, 3 or 21.
A plant belonging to the family Solanaceae which has no traits of dwarfism and a reduced risk for accumulation of glycoalkaloids in which the expression of a gene encoding an enzyme that reduces a double bond between C24 and C25 of the steroid skeleton is suppressed by an artificial procedure, the gene consisting of any one of the DNAs (a) to (c) below:
- (a) DNA consisting of the nucleotide sequence as shown in SEQ ID NO:
- 2, 4 or 22;
- (b) DNA consisting of a nucleotide sequence having 95% or higher sequence identity with the nucleotide sequence as shown in SEQ ID NO:
- 2, 4 or 22 and encoding a protein that reduces a double bond between C24 and C25 of the steroid skeleton;
- and
- (c) DNA encoding a protein consisting of the amino acid sequence as shown in SEQ ID NO:1, 3 or 21.
Dependencies are inferred from the claim text.