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US 9,688,955 B2Granted
Materials for magnetizing cells and magnetic manipulation
AssigneeNano3D Biosciences, Inc.
InventorGlauco R. Souza
Life of this patent
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Abstract
A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.
Claims13
A method of growing a 3D cell culture, said method comprising:
- a) mixing cells with a composition comprising:
- i) a negatively charged nanoparticle;
- ii) a positively charged nanoparticle;
- and
- iii) a support molecule;
- iv) wherein one of said negatively charged nanoparticle or positively charged nanoparticle contains a magnetically responsive element, and wherein said support molecule holds said negatively charged nanoparticle and said positively charged nanoparticle in an intimate admixture forming a fibrous mat-like structure;
- b) incubating said mixture for 1 to 12 hours until the cells become magnetized;
- c) subjecting said magnetized cells to a magnetic field sufficient to levitate said magnetized cells;
- and
- d) growing said levitated cells to make a levitated 3D culture.
7 more elements
Dependencies are inferred from the claim text.