# US 9,688,741 B2 · Elastic hydrogel

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- Number: US 9,688,741 B2
- Document ID: US9688741B2
- Status: Granted
- Kind: B2
- Application number: 14/438,187
- Filed: 2013-10-23
- Published: 2015-10-01
- Granted: 2017-06-27
- Earliest claimed: 2012-10-23
- Assignees: Elastagen Pty. Limited; President and Fellows of Harvard College
- Applicants: Elastagen Pty Ltd; President and Fellows of Harvard College
- Inventors: Nasim Annabi; Anthony Steven Weiss; Ali Khademhosseini
- Web page: https://patentgrep.com/patent/US9688741B2

## CPC

- C07K 14/78: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
- A61L 27/227: {Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24}
- A61L 27/3804: {characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells}
- A61L 27/3826: {Muscle cells, e.g. smooth muscle cells}
- A61L 27/50: Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials}
- A61L 27/52: Hydrogels or hydrocolloids
- A61L 2300/64: Animal cells
- A61L 2430/20: for reconstruction of the heart, e.g. heart valves
- C12N 2533/50: Proteins
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to hydrogels and to the use of hydrogels for repair or restoration of tissue. In particular, the hydrogels of the present invention can be used for the repair or restoration of cardiac tissue.

## Claims

### Claim 1

A hydrogel comprising an acrylated tropoelastin, wherein at least one amine group of the tropoelastin is attached to an acrylate group.

### Claim 2

A hydrogel comprising an acrylated tropoelastin formed by the process of treating a tropoelastin with a compound comprising an acrylate group in conditions for attaching the acrylate group of the compound to an amine of the tropoelastin.

### Claim 3

A hydrogel comprising a cross-linked tropoelastin, the cross-linked tropoelastin comprising:
a plurality of tropoelastin monomers or multimers; and
a linker in the form of a compound having an acrylate group, wherein the acrylate group of the compound is attached to an amine group of the tropoelastin for cross-linking the monomers or multimers.

### Claim 4

A hydrogel comprising an acrylated tropoelastin, wherein about 30% to about 50% of the lysine residues of the tropoelastin are attached to an acrylate group.

### Claim 5 (depends on 1)

A tissue-engineered construct comprising a hydrogel of claim 1.

### Claim 6 (depends on 2)

A tissue-engineered construct comprising a hydrogel of claim 2.

### Claim 7 (depends on 3)

A tissue-engineered construct comprising a hydrogel of claim 3.

### Claim 8 (depends on 4)

A tissue-engineered construct comprising a hydrogel of claim 4.

### Claim 9 (depends on 1)

A device, support or scaffold comprising a hydrogel according to claim 1.

### Claim 10 (depends on 2)

A device, support or scaffold comprising a hydrogel according to claim 2.

### Claim 11 (depends on 3)

A device, support or scaffold comprising a hydrogel according to claim 3.

### Claim 12 (depends on 4)

A device, support or scaffold comprising a hydrogel according to claim 4.