Method for measurement of peptidic degradation products of a proteolytic cascade in blood samples
AssigneeAttoquant Diagnostics GmbH
InventorsMarko Poglitsch, Cornelia Schwager, Hans Loibner, Manfred Schuster
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Abstract
The invention discloses a method for measurement of peptidic degradation products of a proteolytic cascade (for example, the renin-angiotensin system (RAS) and the bradykinin system) in biological samples, especially blood samples, wherein the sample is incubated until a steady state equilibrium is reached for at least one peptidic degradation product involved in said proteolytic cascade and wherein said at least one peptidic degradation product in steady state equilibrium of the proteolytic cascade is quantified in the sample.
Claims19
A method for measurement of peptidic degradation products of a proteolytic cascade comprising at least two consecutive proteolytic reactions in a biological sample comprising:
- incubating a biological sample ex vivo until the actual overall degradation rate of at least one peptidic degradation product is equal to the actual overall formation rate of the at least one peptidic degradation product and a steady state equilibrium is reached for the at least one peptidic degradation product involved in the proteolytic cascade;
- and
- quantifying at least one peptidic degradation product in a steady state equilibrium concentration in the sample,
- wherein the method further comprises adding one or more protease inhibitor and/or one or more chaotropic agent after the incubation until a steady state equilibrium is reached for at least one peptidic degradation product, and wherein substrates of any enzyme(s) involved in the proteolytic cascade are not added before and/or during the incubation until a steady state equilibrium is reached.
Dependencies are inferred from the claim text.