Mutant polypeptides and uses thereof
AssigneeInvista North America S.A.R.L.
InventorsEric Althoff, Nadia Kadi, Mihai Luchian Azoitei, Yih-En A Ban, Daniela Grabs-Röthlisberger, Alexander Pisarchik, Alexandre Zanghellini, Adriana L. Botes
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Abstract
The present disclosure provides novel polypeptides with 3-buten-2-ol dehydratase activity, polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene, and crystal structure data for one of such polypeptides. Methods of making and using the polypeptides and their related crystal structure data are also provided.
Claims11
A composition comprising bioderived 1,3-butadiene in the presence of a polypeptide comprising the amino acid sequence of SEQ ID NO:462, wherein SEQ ID NO:462 is a triple mutant of SEQ ID NO:1 carrying the following three mutations:
- F382W, L328V, and I187M;
- or of a signal-peptide-less version thereof (SEQ ID NO:460).
A composition comprising bioderived isoprene in the presence of a polypeptide comprising the amino acid sequence of SEQ ID NO:462, wherein SEQ ID NO:462 is a triple mutant of SEQ ID NO:1 carrying the following three mutations:
- F382W , L328V , and I187M;
- or of a signal-peptide-less version thereof (SEQ ID NO:460).
A composition comprising bioderived 1,3-butadiene in the presence of a polypeptide comprising a sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:462, wherein the polypeptide carries at least all three F382W, L328V, and I187M mutations;
- or a signal-peptide-less version thereof, wherein the polypeptide has 3-buten-2-ol dehydratase activity.
A composition comprising bioderived isoprene in the presence of a polypeptide comprising a sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:462, wherein the polypeptide carries at least all three F382W, L328V, and I187M mutations;
- or a signal-peptide-less version thereof, wherein the polypeptide has 3-methyl-3-buten-2-ol dehydratase activity.
A composition comprising bioderived 1,3-butadiene in the presence of a trace amount of a recombinant host cell or recombinant organism that expresses a polypeptide comprising a sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:462, wherein the polypeptide carries at least all three F382W, L328V, and I187M mutations;
- or a signal-peptide-less version thereof, wherein the polypeptide has 3-buten-2-ol dehydratase activity.
A composition comprising bioderived isoprene in the presence of a trace amount of a recombinant host cell or recombinant organism that expresses a polypeptide comprising a sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:462, wherein the polypeptide carries at least all three F382W, L328V, and I187M mutations;
- or a signal-peptide-less version thereof, wherein the polypeptide has 3-methyl-3-buten-2-ol dehydratase activity.
Dependencies are inferred from the claim text.