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US 9,678,038 B2Granted
Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
AssigneeThe Trustees of Princeton University
InventorsRobert H. Austin, Zhaoning Yu, Jonas O. Tegenfeldt, Stephen Y. Chou, Han Cao
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Abstract
Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.
Claims28
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the macromolecule is DNA and the detected signals are correlated to the base pair sequence of said DNA;
- and
- wherein the nanochannel is surmounted by sealing material to render the nanochannel at least substantially enclosed.
5 more elements
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the macromolecule is DNA and the detected signals are correlated to the base pair sequence of said DNA;
- and
- wherein the at least one macromolecule has an elongated length in nanochannel of greater than 150 nanometers.
5 more elements
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the macromolecule is DNA and the detected signals are correlated to the base pair sequence of said DNA;
- and
- wherein the at least one macromolecule is a DNA fragment from a restriction fragment length polymorphism analysis.
5 more elements
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the macromolecule is DNA and the detected signals are correlated to the base pair sequence of said DNA;
- and
- wherein the at least one macromolecule is at least one chromosome.
5 more elements
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- and
- illuminating the at least one macromolecule within the nanochannel;
- wherein the macromolecule is DNA and the detected signals are correlated to the base pair sequence of said DNA.
5 more elements
A method of maintaining at least one macromolecule in an elongated state, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- and
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- wherein the at least one macromolecule is a DNA fragment from a restriction fragment length polymorphism analysis.
1 more element
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the at least one macromolecule is a DNA fragment from a restriction fragment length polymorphism analysis.
3 more elements
A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- detecting at least one signal transmitted from the at least one elongated macromolecule within the nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule;
- wherein the at least one macromolecule is at least one chromosome.
3 more elements
A method of maintaining at least one macromolecule in an elongated state, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in an elongated state within the nanochannel;
- and
- maintaining the at least one macromolecule in an elongated state within the nanochannel without application of a shear force;
- wherein the at least one macromolecule is at least one chromosome.
1 more element
Dependencies are inferred from the claim text.