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US 9,677,106 B2Granted
Method for producing gamma-glutamylvalylglycine or a salt thereof
AssigneeAjinomoto Co., Inc.
InventorsHiroyuki Nozaki, Isao Abe, Jun Takakura, Rie Takeshita, Hideyuki Suzuki, Shunichi Suzuki
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Abstract
A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
Claims20
A method for producing γ-Glu-Val-Gly, comprising:
- reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase such that γ-Glu-Val-Gly is generated,
- wherein the γ-glutamyltransferase comprises a large subunit and a small subunit,
- the large subunit has an amino acid sequence having no less than 90% sequence identity to the amino acid sequence of the positions 26 to 390 of the amino acid sequence of SEQ ID NO:
- 2,
- the small subunit has an amino acid sequence having no less than 90% sequence identity to the amino acid sequence of the positions 391 to 580 of the amino acid sequence of SEQ ID NO:
- 2, and
- the tyrosine at the position corresponding to position 444 of SEQ ID NO:
- 2 is modified by a substitution.
5 more elements
Dependencies are inferred from the claim text.