Diterpene synthases and method for producing diterpenoids
AssigneeThe University of British Columbia
InventorsJoerg Bohlmann, Philipp Zerbe
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Abstract
Provided herein are diterpene synthases (diTPS) and methods for producing diterpenoids. Also provided herein are nucleic acid sequences encoding diTPS, diTPS amino acid sequences, diTPS proteins, vectors, cells, transgenic organisms, uses, compositions, methods, processes, and kits thereof.
Claims16
An isolated nucleic acid molecule, comprising a sequence of nucleotides encoding a cis-abienol synthase (CAS) polypeptide having an amino acid sequence that has at least 85% sequence identity with a polypeptide whose sequence is set forth in one of SEQ ID NOS:7, 50 and 55 or an active fragment thereof, wherein:
- the encoded polypeptide or active fragment catalyzes the formation of cis-abienol from geranylgeranyl diphosphate (GGPP);
- and
- the isolated nucleic acid molecule is cDNA.
A host cell, comprising a nucleic acid molecule encoding a cis-abienol synthase (CAS) polypeptide having an amino acid sequence that has at least 85% sequence identity with a polypeptide whose sequence is set forth in one of SEQ ID NOS:7, 50 and 55 or an active fragment thereof, wherein:
- the encoded polypeptide or active fragment catalyzes the formation of cis-abienol from geranylgeranyl diphosphate (GGPP);
- and the encoded cis-abienol synthase is heterologous to the host cell.
Dependencies are inferred from the claim text.