Adeno associated virus plasmids and vectors
AssigneesOregon Health & Science University, University of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsHiroyuki Nakai, Kei Adachi
Life of this patent
Estimating the term…
Abstract
Disclosed herein are adeno associated viral plasmids and viral vectors. Also disclosed are methods of using adeno associated viral vectors.
Claims15
An adeno associated virus plasmid comprising:
- a polynucleotide that encodes a protein with the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence having at least about 60%, at least about 75%, at least about 80%, at least about 90%, or at least about 95% sequence identity to SEQ ID NO:
- 1, wherein the protein further comprises a mutation at at least one of L380, T381, L382, N383, I440, D441, Y446, L447, I450, T451, V465, S469, N470, M471, Q474, G475, Y484, R485, E500, W503, P504, R514, N515, S516, and L517.
An adeno associated virus plasmid comprising:
- a polynucleotide that encodes a protein with the amino acid sequence of SEQ ID NO:
- 4 or an amino acid sequence having at least about 60%, at least about 75%, at least about 80%, at least about 90%, or at least about 95% sequence identity to SEQ ID NO:
- 4, the protein further comprises a mutation at at least one of Q464, A467, D469, I470, R471, D472, S474, S501, and D514.
An adeno associated virus plasmid comprising:
- a polynucleotide that encodes a protein with an amino acid sequence selected from at least one of SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 15, SEQ ID NO:
- 16, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:20, SEQ ID NO:
- 21, SEQ ID NO:
- 23, SEQ ID NO:
- 24, and SEQ ID NO:
- 25.
A method of delivering a polynucleotide that encodes a protein of interest to a tissue in a subject, the method comprising:
- administering an effective amount of an adeno associated virus vector to the subject, wherein the adeno associated virus vector comprises an adeno associated virus plasmid comprising:
- a polynucleotide that encodes a protein with the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence having at least about 90% or at least about 95% sequence identity to SEQ ID NO:
- 1, wherein the protein further comprises a mutation at at least one of L380, T381, L382, N383, I440, D441, Y446, L447, T450, I451, V465, S469, N470, M471, Q474, G475, Y484, R485, E500, W503, P504, R514, N515, S516, and L517.
A method of delivering a polynucleotide that encodes a protein of interest to a tissue in a subject, the method comprising:
- administering an effective amount of an adeno associated virus vector to the subject, wherein the adeno associated virus vector comprises an adeno associated virus plasmid comprising:
- a polynucleotide that encodes a protein with the amino acid sequence of SEQ ID NO:
- 4 or an amino acid sequence having at least about 90%, or at least about 95% sequence identity to SEQ ID NO:
- 4, the protein further comprises a mutation at at least one of Q464, A467, D469, I470, R471, D472, S474, S501, and D514.
An adeno associated virus plasmid comprising:
- a polynucleotide that encodes a protein with the amino acid sequence of SEQ ID NO:
- 4 or an amino acid sequence having at least about 60%, at least about 75%, at least about 80%, at least about 90%, or at least about 95% sequence identity to SEQ ID NO:
- 4, further comprising a Y500E mutation.
An adeno associated virus plasmid comprising:
- a polynucleotide that encodes a protein with the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence having at least about 60%, at least about 75%, at least about 80%, at least about 90%, or at least about 95% sequence identity to SEQ ID NO:
- 1, further comprising at least one of a F501A mutation, a G505A mutation, and a Q590A mutation.
Dependencies are inferred from the claim text.