US 9,677,082 B2Granted

Haploid induction compositions and methods for use therefor

AssigneeSyngenta Participations AG

InventorsSatya Chintamanani, Brent Delzer, Michael L Nuccio, Robert Arthur Dietrich, Suresh Babu Kadaru, Todd Lee Warner, William Paul Bullock, Timothy Kelliher

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Abstract

Provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit haploid induction (HI); methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

Claims2

Dependent claims

Method of creating a new haploid inducer maize plant with a silenced patatin-like phospholipase 2A, comprising transcribing a polynucleotide sequence capable of silencing the patatin-like phospholipase 2A in maize, wherein said polynucleotide sequence comprises a first sequence selected from the group consisting of:

a) a polynucleotide sequence comprising the nucleic acid sequence set forth in SEQ ID NO:
37 or the complement thereof,
b) a functional fragment comprising at least 22 contiguous bases of SEQ ID NO:
37 or the complement thereof, and
c) a polynucleotide sequence having at least 95% sequence identity as determined using the BLASTN alignment tool to the nucleic acid sequence set forth in SEQ ID NO:
37 or the complement thereof,
and a second sequence that is the complement of the first sequence, wherein the polynucleotide sequence expresses a double-stranded ribonucleotide sequence which silences the patatin-like phospholipase 2A when contacted with a maize plant and thus creates a new haploid inducer maize plant.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 14/212,504Filed 14 Mar 2014 · us provisional application
Priority: 15 Mar 2013
Published: US 2017/0067067 A19 Mar 2017
Granted: 13 Jun 2017
Term ends: Not estimatedThe source records don’t support an estimate.

Classification18 codes

C12N 15/8216
For plant cells , e.g. plant artificial chromosomes: methods for controlling, regulating or enhancing expression of transgenes in plant cells
A01H 1/045
Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection: using molecular markers
A01H 1/08
Processes for producing mutations, e.g. treatment with chemicals or with radiation: methods for producing changes in chromosome number
C12N 9/18
Acting on ester bonds: carboxylic ester hydrolases