Microorganisms and methods for production of 4-hydroxybutyrate, 1,4-butanediol and related compounds
AssigneeGenomatica, Inc.
InventorsPriti Pharkya, Anthony P. Burgard, Stephen J. Van Dien, Robin E. Osterhout, Mark J. Burk, John D. Trawick, Michael P. Kuchinskas, Brian Steer
Life of this patent
Estimating the term…
Abstract
The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.
Claims12
A non-naturally occurring microbial organism, said microbial organism having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein said microbial organism comprises genetic modifications, said genetic modifications comprising:
- (A) a genetic modification that increases expression of phosphoenolpyruvate carboxylase;
- (B) a genetic modification that increases expression of alpha-ketoglutarate dehydrogenase;
- and
- (C) a genetic modification that decreases expression of an acyl coenzyme A thioesterase.
Dependencies are inferred from the claim text.