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US 9,676,837 B2Granted
Collagen 7 and related methods
AssigneeShire Human Genetic Therapies, Inc.
InventorsMalini Viswanathan, Mark DeSouza
Life of this patent
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Abstract
Disclosed are methods of making collagen 7, or functional fragments thereof, as well as collagen 7, and functional fragments thereof produced by such methods, nucleic acids encoding collagen 7, and functional fragments thereof, as well as vectors and host cells comprising such nucleic acids.
Claims15
A method of making human collagen 7, or a functional fragment of human collagen 7, comprising:
- providing a cell, which comprises an exogenously introduced nucleic acid that encodes human collagen 7, or a functional fragment thereof, wherein said cell is recombinantly manipulated to express one or more polypeptides that increase expression of human collagen 7, or a functional fragment thereof, and wherein the one or more polypeptides comprises prolidase;
- and
- culturing said cell under conditions sufficient for the production of human collagen 7, or the functional fragment of human collagen 7, and prolidase, thereby making human collagen 7, or the functional fragment thereof.
A cell comprising:
- a first expression vector comprising a first nucleic acid sequence that encodes human collagen 7 or a functional fragment thereof,
- a second expression vector comprising a second nucleic acid sequence that encodes one or more polypeptides that increase expression of human collagen 7, wherein said one or more polypeptides comprises prolidase, and
- optionally a third expression vector comprising a third nucleic acid sequence that encodes glycosyl transferase.
A cell comprising:
- a vector comprising a first nucleic acid sequence that encodes human collagen 7 or a functional fragment thereof, a second nucleic acid sequence that encodes prolidase, and optionally a third nucleic acid sequence that encodes glycosyl transferase.
A method of making a cell suitable for expressing human collagen 7, or a functional fragment thereof, comprising
- recombinantly manipulating said cell to express recombinant human collagen 7, or the functional fragment thereof;
- and
- recombinantly manipulating said cell to express one or more polypeptides that increase expression of human collagen 7, wherein the one or more polypeptides comprises recombinant prolidase;
- thereby making a cell suitable for expressing recombinant human collagen 7, or the functional fragment thereof.
1 more element
Dependencies are inferred from the claim text.