Methods and means to modify a plant genome
AssigneeBayer CropScience AG
InventorsKathleen D'Halluin, Jan Dittgen, Stefan Jansens, Frank Meulewaeter, Koen Weterings, Hal Moser, Thomas Wilde, Ruediger Hain, Udo Bickers, Linda Trolinder, Gary Henniger
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Abstract
Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1× of at least one HPPD inhibitor, and methods for making such plants.
Claims28
A cotton plant cell, plant part, plant, or seed comprising a chimeric gene comprising
- (a) a nucleic acid sequence encoding a protein having HPPD activity, wherein said protein has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 21 and has a tryptophan at a position corresponding to position 336 of SEQ ID NO:
- 19, and wherein said protein provides to said plant tolerance to a field dose of at least 1× of at least one HPPD inhibitor, said sequence operably linked to
- (b) a plant expressible promoter, which is a 35S or CsVMV promoter.
A method of producing a cotton plant, or plant cell, plant part, or seed thereof that is tolerant to a field dose of at least 1× of at least one HPPD inhibitor, comprising introducing into the cotton plant, or plant cell, plant part, or seed thereof a nucleic acid sequence encoding a protein having HPPD activity, wherein said protein has a tryptophan at a position corresponding to position 336 of SEQ ID NO:
- 19.
Dependencies are inferred from the claim text.