Genetically engineered yeast cells
AssigneeFirmenich SA
InventorsJens Nielsen, Verena Siewers, Yun Chen, Laurent Daviet, Michel Schalk
Life of this patent
Estimating the term…
Abstract
The present invention relates to yeast cells producing high levels of acetoacetyl-CoA. It also relates to a method for making such yeast cells and to the use of such yeast cells in a method for producing acetyl-CoA derived products.
Claims11
A yeast cell modified by overexpression of an aldehyde dehydrogenase, and an acetyl-CoA synthetase (ACS), an alcohol dehydrogenase and an acetoacetyl-CoA synthase, and characterized in that a gene encoding a peroxisomal citrate synthase or a gene encoding a cytosolic malate synthase has been deleted from the yeast cell genome.
A method of making a modified yeast cell comprising over-expressing an aldehyde dehydrogenase, an acetyl-CoA synthase (ACS), an alcohol dehydrogenase and an acetoacetyl-CoA synthase in a yeast cell, and deleting a gene encoding a peroxisomal citrate synthase or a gene encoding a cytosolic malate synthase from the yeast cell genome.
Dependencies are inferred from the claim text.