Polypeptides having peroxygenase activity
AssigneeNovozymes A/S
InventorsSara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
Life of this patent
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Abstract
The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims20
A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted, linear or branched, alkane or saturated fatty acid having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3, comprising contacting said alkane or said saturated fatty acid with hydrogen peroxide and an isolated polypeptide having peroxygenase activity and comprising an amino acid sequence having at least 95% sequence identity to the sequence of amino acids 1 to 244 of SEQ ID NO:
- 2, wherein said alkane or said saturated fatty acid is optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxyl, carboxyl, amino, nitro, cyano, thiol, sulphonyl, formyl, acetyl, methoxy, ethoxy, phenyl, benzyl, xylyl, carbamoyl and sulfamoyl.
A method for introducing a keto group at the second or third carbon of at least two ends of a substituted or unsubstituted, linear or branched, alkane or saturated fatty acid having at least five carbons and having at least one hydrogen attached to said second or third carbon, comprising contacting said alkane or said saturated fatty acid with hydrogen peroxide and an isolated polypeptide having peroxygenase activity and comprising an amino acid sequence having at least 95% sequence identity to the sequence of amino acids 1 to 244 of SEQ ID NO:
- 2, wherein said alkane or said saturated fatty acid is optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxyl, carboxyl, amino, nitro, cyano, thiol, sulphonyl, formyl, acetyl, methoxy, ethoxy, phenyl, benzyl, xylyl, carbamoyl and sulfamoyl.
Dependencies are inferred from the claim text.