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US 9,663,810 B2Granted
Methods of cell culture
AssigneeMomenta Pharmaceuticals, Inc.
InventorHolly Prentice
Life of this patent
Estimating the term…
Abstract
Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using putrescine, are described.
Claims24
A method of producing a recombinant protein preparation having a target value of fucosylated glycans, the method comprising:
- (a) providing a cell genetically engineered to express a recombinant protein;
- (b) culturing the cell in a culture medium comprising 0.1 mg/L to 10 mg/L putrescine under conditions in which the cell expresses the recombinant protein;
- and
- (c) harvesting a preparation of the recombinant protein produced by the cell that meets the target value of the fucosylated glycans
- wherein the target value of fucosylated glycans is a level at least 10% higher than a level of fucosylated glycans in a preparation produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine.
2 more elements
A method of producing a recombinant protein preparation, the method comprising:
- (a) providing a target value of fucosylated glycans;
- (b) providing a cell genetically engineered to express a recombinant protein;
- (c) culturing the cell in a culture medium comprising 0.1 mg/L to 10 mg/L putrescine under conditions in which the cell expresses the recombinant protein;
- (d) harvesting a preparation of the recombinant protein produced by the cell;
- and
- (e) formulating the preparation into a drug product if the preparation meets the target value of fucosylated glycans
- wherein the target value of fucosylated glycans is a level at least 10% higher than a level of fucosylated glycans in a preparation produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine.
4 more elements
A method of increasing a level of fucosylated glycans in a recombinant protein preparation, the method comprising:
- (a) providing a cell genetically engineered to express a recombinant protein;
- (b) culturing the cell in a culture medium comprising 0.1 mg/L to 10 mg/L putrescine under conditions in which the cell expresses the recombinant protein;
- and
- (c) harvesting a preparation of the recombinant protein produced by the cell, wherein the preparation has a level of fucosylated glycans, that is at least 10% higher than a level of fucosylated glycans in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine.
1 more element
Dependencies are inferred from the claim text.