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US 9,663,801 B2Granted
Methods of producing four carbon molecules
AssigneeInvista North America S.A.R.L.
InventorsPaul S. Pearlman, Changlin Chen, Adriana L. Botes
Life of this patent
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Abstract
Disclosed are methods for producing butadiene from one or more of several diverse feedstocks including bioderived feedstocks, renewable feedstocks, petrochemical feedstocks and natural gas.
Claims12
A method for producing 1,3-butadiene comprising:
- fermenting a fermentable feedstock in the presence of a genetically modified microorganism under conditions to produce 1,3-butadiene, wherein said genetically modified microorganism comprises heterologous nucleic acids encoding a dehydratase enzyme classified under EC 4.2.1.- and a desaturase enzyme selected from the group consisting of cytochrome P450 enzymes, clavaminate synthase 2 enzymes, and enzymes classified under EC 1.14.19-, EC 1.14.99-, EC 1.3.1-;
- and isolating said 1,3-butadiene.
A method of producing 1,3-butadiene comprising:
- contacting butenol with a dehydratase enzyme classified under EC 4.2.1- to dehydrate said butenol to produce 1,3-butadiene, wherein said butenol is selected from the group consisting of 1-buten-3-ol, 1-buten-4-ol, 2-buten-1-ol, 2-buten-2-ol, 1-buten-1-ol, and 1-buten-2-ol.
Dependencies are inferred from the claim text.