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US 9,663,799 B2Granted
Hydrocarbon synthase gene and use thereof
AssigneeToyota Jidosha Kabushiki Kaisha
InventorsMasayoshi Muramatsu, Masakazu Ito
Life of this patent
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Abstract
A hydrocarbon synthase gene encoding protein having excellent capacity to synthesize a hydrocarbon such as alkane and novel functions is provided. The gene encodes a protein comprising an amino acid sequence comprising a motif sequence shown in SEQ ID NO: 1 and having activity of synthesizing a hydrocarbon with a carbon number one less than that of an aldehyde compound from the aldehyde compound.
Claims6
A method for producing a hydrocarbon, said method comprising incubating together:
- a protein having an activity of synthesizing, from an aldehyde compound, a hydrocarbon with a carbon number one less than that of the aldehyde compound, wherein said protein is selected from the group consisting of (a) and (b):
- (a) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 12, 26, 66, 72, 76, 80, 90, 92, 94, 98, 112, 116, 122 and 144;
- and
- (b) a protein comprising an amino acid sequence having 90% or more identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 12, 26, 66, 72, 76, 80, 90, 92, 94, 98, 112, 116, 122 and 144, and having an activity of synthesizing, from an aldehyde compound, a hydrocarbon with a carbon number one less than that of the aldehyde compound;
- a coenzyme involved in said activity of said protein, wherein the coenzyme is reduced nicotinamide adenine dinucleotide (NADH) or nicotinamide adenine dinucleotide phosphate, and
- an aldehyde compound that serves as a substrate for said activity of said protein,
- to thereby synthesize a hydrocarbon with a carbon number one less than that of the aldehyde compound.
6 more elements
Dependencies are inferred from the claim text.