Standard material for a particle analyzer

A method for judging an abnormality of a particle analyzer which comprises a measuring sample preparation unit configured to prepare a measuring sample by mixing a urine sample with a fluorescence dye, a light source configured to irradiate the measuring sample with a light and a fluorescence detector configured to detect fluorescence from the measuring sample, comprising steps of:

• providing a polyacrylamide particle which is fluorescently unstained, a hydrophilic vinyl polymer particle which is fluorescently unstained, and a fluorescent latex particle;
• contacting, with the measuring sample preparation unit, a first fluorescence dye with the polyacrylamide particle and the hydrophilic vinyl polymer particle to fluorescently stain the polyacrylamide particle and the hydrophilic vinyl polymer particle,
• the polyacrylamide particle when stained fluorescently being similar in fluorescence intensity to an epithelial cell in the urine sample which is fluorescently stained with the first fluorescence dye, and
• the hydrophilic vinyl polymer particle when stained fluorescently being similar in fluorescence intensity to a columnar cell in the urine sample which is fluorescently stained with the first fluorescence dye,
• contacting, with the measuring sample preparation unit, a second fluorescence dye with the fluorescent latex particle,
• the fluorescent latex particle being substantially not able to be stained by the first and second fluorescence dyes, and
• the fluorescent latex particle being similar in fluorescence intensity to bacteria which are stained by the measuring sample preparation unit;
• irradiating with the light source a light to the polyacrylamide particle, the hydrophilic vinyl polymer particle and the fluorescent latex particle;
• detecting with the fluorescence detector a first fluorescence from polyacrylamide particle, a second fluorescence from the hydrophilic vinyl polymer particle, and a third fluorescence from the fluorescent latex particle;
• detecting a first fluorescence intensity based on the first fluorescence, a second fluorescence intensity based on the second fluorescence, and a third fluorescence intensity based on the third fluorescence,
• comparing the first fluorescence intensity with a condition corresponding to expected fluorescence intensity of the polyacrylamide particle stained fluorescently with the first fluorescence dye defined by predetermined upper and lower limit values;
• comparing the second fluorescence intensity with a condition corresponding to expected fluorescence intensity of the hydrophilic vinyl polymer particle stained fluorescently with the first fluorescence dye defined by predetermined upper and lower limit values;
• comparing the third fluorescence intensity with a condition corresponding to expected fluorescence intensity of the fluorescent latex particle defined by predetermined upper and lower limit values;
• and
• judging an abnormality of the particle analyzer when at least one fluorescence intensity selected from the group consisting of the first, second and third fluorescence intensities is outside the predetermined upper and lower limit values.

A method for judging an abnormality of a particle analyzer which comprises a measuring sample preparation unit configured to prepare a measuring sample by mixing a urine sample with a fluorescence dye, a light source configured to irradiate the measuring sample with a light, a scattered light detector configured to detect scattered light from the measuring sample, and a fluorescence detector configured to detect fluorescence from the measuring sample, comprising steps of:

• providing a polyacrylamide particle which is fluorescently unstained, a hydrophilic vinyl polymer particle which is fluorescently unstained, and a fluorescent latex particle;
• contacting, with the measuring sample preparation unit, a first fluorescence dye with the polyacrylamide particle and the hydrophilic vinyl polymer particle to fluorescently stain the polyacrylamide particle and the hydrophilic vinyl polymer particle,
• the polyacrylamide particle when stained fluorescently being similar in fluorescence intensity to an epithelial cell in the urine sample which is fluorescently stained with the first fluorescence dye, and
• the hydrophilic vinyl polymer particle when stained fluorescently being similar in fluorescence intensity to a columnar cell in the urine sample which is fluorescently stained with the first fluorescence dye,
• contacting, with the measuring sample preparation unit, a second fluorescence dye with the fluorescent latex particle,
• the fluorescent latex particle being substantially not able to be stained by the first and second fluorescence dyes, and
• the fluorescent latex particle being similar in fluorescence intensity to bacteria which are stained by the measuring sample preparation unit;
• irradiating with the light source a light to the polyacrylamide particle, the hydrophilic vinyl polymer particle and the fluorescent latex particle;
• detecting with the fluorescence detector a first fluorescence from polyacrylamide particle, a second fluorescence from the hydrophilic vinyl polymer particle, and a third fluorescence from the fluorescent latex particle;
• detecting with the scattered light detector a first scattered light from polyacrylamide particle, a second scattered light from the hydrophilic vinyl polymer particle, and a third scattered light from the fluorescent latex particle;
• detecting a first fluorescence intensity based on the first fluorescence, a second fluorescence intensity based on the second fluorescence, a third fluorescence intensity based on the third fluorescence, a first scattered light intensity based on the first scattered light, and a second scattered light intensity based on the second scattered light,
• comparing the first fluorescence intensity with a condition corresponding to expected fluorescence intensity of the polyacrylamide particle stained fluorescently with the first fluorescence dye defined by predetermined upper and lower limit values;
• comparing the second fluorescence intensity with a condition corresponding to expected fluorescence intensity of the hydrophilic vinyl polymer particle stained fluorescently with the first fluorescence dye defined by predetermined upper and lower limit values;
• comparing the third fluorescence intensity with a condition corresponding to expected fluorescence intensity of the fluorescent latex particle defined by predetermined upper and lower limit values;
• comparing the first scattered light intensity with a condition corresponding to expected scattered light intensity of the polyacrylamide particle defined by predetermined upper and lower limit values;
• comparing the second scattered light intensity with a condition corresponding to expected scattered light intensity of the hydrophilic vinyl polymer particle defined by predetermined upper and lower limit values;
• comparing the third scattered light intensity with a condition corresponding to expected scattered light intensity of the fluorescent latex particle defined by predetermined upper and lower limit values;
• and
• judging an abnormality of the particle analyzer when at least one selected from the group consisting of the first, second and third fluorescence intensities and the first and second scattered light intensities is outside the predetermined upper and lower limit values.