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US 9,657,316 B2Granted
Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,4-butanediol related thereto
AssigneeGenomatica, Inc.
InventorsAnthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Abstract
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.
Claims28
A non-naturally occurring microbial organism comprising:
- (A) a methanol metabolic pathway, wherein said non-naturally occurring microbial organism comprises exogenous nucleic acids encoding a methanol metabolic pathway enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of methanol, or metabolize methanol as a carbon source for biosynthesis of 1,4-butanediol (BDO), wherein said methanol metabolic pathway comprises:
- (i) a methanol methyltransferase and a methylenetetrahydrofolate reductase;
- (ii) a methanol dehydrogenase;
- or
- (iii) a methanol dehydrogenase and a formaldehyde activating enzyme;
- and
- (B) a 1,4-butanediol (BDO) pathway comprising at least two exogenous nucleic acids each encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO.
4 more elements
Dependencies are inferred from the claim text.