Brassica AHAS genes and gene alleles that provide resistance to imidazolinone herbicides
AssigneeViterra, Inc.
InventorsKening Yao, Derek A. Potts, Bradley D. Leibel, Daryl R. Males
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Abstract
Plants, plant parts and plant seeds that are resistant to imidazolinone herbicides are provided. Plants are disclosed that contain a mutation in an AHAS gene. Specifically, plants are disclosed that contain a mutant AHAS gene allele of the Brassica juncea B genome. Two B. juncea AHAS gene sequences (BjAHAS-a and BjAHAS-b) and one B. nigra AHAS gene sequence (BngrAHAS) are disclosed. The sequence of the mutant allele, BjAHAS-bR, is also disclosed. Various methods are disclosed that include creation of mutant B. juncea lines, selection for herbicide resistant lines and determining the presence of the BjAHAS-bR mutant allele after crosses.
Claims18
A Brassica juncea plant obtained by a process comprising crossing a plant of line J04E-0044, a representative sample of seed of the line having been deposited under ATCC Accession No. PTA-6324, with another Brassica variety,
- wherein the plant comprises a B-genome having a Brassica juncea acetohydroxyacid synthase (AHAS) nucleic acid at the AHAS-b locus thereof, the nucleic acid encoding an herbicide resistant AHAS polypeptide comprising amino acid residues 114 to 655 of SEQ ID NO:15, said plant exhibiting increased tolerance to an AHAS-inhibiting herbicide as compared to that of a corresponding wild-type plant.
A method of producing Brassica juncea plant lines wherein the method comprises:
- a) making a cross involving a first parent and a second parent plant, wherein the first plant is a plant of line J04E-0044, a representative sample of seed of the line having been deposited under ATCC Accession No. PTA-6324 and comprises a B-genome having a Brassica juncea acetohydroxyacid synthase (AHAS) nucleic acid at the AHAS-b locus thereof, the nucleic acid encoding an herbicide resistant AHAS a polypeptide comprising amino acid residues 114 to 655 of SEQ ID NO:15;
- b) obtaining seeds from the cross of step (a);
- c) growing fertile plants from such seeds;
- d) identifying progeny plants that are herbicide resistant and that comprise a polynucleotide encoding a polypeptide comprising amino acid residues 114 to 655 of SEQ ID NO:15;
- and
- e) obtaining progeny seeds from plants identified in step (d), wherein the Brassica juncea plant lines are commercial Brassica juncea plant lines.
Dependencies are inferred from the claim text.