Back to results
US 9,657,227 B2Granted
Preservation of cell-free RNA in blood samples
AssigneeStreck, Inc.
InventorM. Rohan Fernando
Life of this patent
Estimating the term…
Abstract
A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.
Claims20
A direct blood draw tube for stabilization of cell-free nucleic acid within a plasma sample comprising:
- a composition including:
- a) one or more preservative agents including imidazolidinyl urea;
- b) one or more enzyme inhibitors;
- and
- c) one or more solvents;
- and
- wherein the one or more enzyme inhibitors are selected from the group consisting of EDTA, aurintricarboxylic acid, and combinations thereof;
- wherein an amount of the one or more preservative agents relative to an amount of the one or more enzyme inhibitors is about 3 to about 6 parts by weight of preservative agent to about 2 parts by weight of enzyme inhibitors;
- wherein the composition contains less than, or about 30% by weight of the one or more enzyme inhibitors;
- wherein the composition includes some formaldehyde but less than 500 parts per million of formaldehyde;
- and
- wherein a concentration of the preservative agent is less than, or about 1000 g/l of the composition;
- and
- wherein the direct blood draw tube including the composition and a blood sample located therein is storable at room temperature.
11 more elements
Dependencies are inferred from the claim text.