Production of fatty alcohols from engineered microorganisms
AssigneeCodexis, Inc.
InventorsJonathan Vroom, Svetlana Balatskaya, Yoram Barak, Louis Clark, Trish Choudhary, Kristian Karlshoej, Fernando Valle, Kaman Chan
Life of this patent
Estimating the term…
Abstract
Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a β-ketoacyl acyl carrier protein synthase.
Claims33
An engineered prokaryotic cell comprising two recombinant polynucleotides, the first polynucleotide encodes a heterologous fatty alcohol forming reductase (FAR) comprising the amino acid sequence of SEQ ID NO:
- 8, and the second polynucleotide encodes a β-ketoacyl acyl carrier protein synthase III (FabH).
A vector comprising a first polynucleotide encoding a FAR comprising the amino acid sequence of SEQ ID NO:
- 8, and a second polynucleotide encoding a FabH, wherein the first and second polynucleotides are operably linked to a promoter that is functional in a prokaryotic host cell.
A recombinant bacterial culture that produces a composition of fatty alcohols comprising at least 60% C12, C14 and C16 fatty alcohols, wherein the recombinant bacterial culture comprises an engineered bacterial microorganism comprising a gene encoding a heterologous FAR comprising the amino acid sequence of SEQ ID NO:
- 8 and a gene encoding a heterologous FabH comprising an amino acid sequence at least 90% sequence identity to SEQ ID NO:
- 10.
A method of shifting the C12, and C16 fatty alcohols carbon chain length profile in a fatty alcohol composition produced by an engineered bacterial cell comprising a polynucleotide encoding a heterologous FAR having the amino acid sequence of SEQ ID NO:
- 8, said method comprises introducing a polynucleotide encoding a FabH having at least 90% sequence identity to SEQ ID NO:
- 10 into the engineered bacterial cell to produce newly engineered bacterial cell, wherein the composition of C12 fatty alcohols produced by the newly engineered bacterial cell has been increased by 25% and the composition of C16 fatty alcohols has been decreased by at least 10% as compared to the engineered bacterial cell.
Dependencies are inferred from the claim text.