Methods and compositions to protect aquatic invertebrates from disease
AssigneeHarrisvaccines, Inc.
InventorsJohn Dustin Loy, Lyric Colleen Bartholomay, Delbert Linn Harris, Ed Scura, Kurt Iver Kamrud
Life of this patent
Estimating the term…
Abstract
Compositions and methods of protecting aquatic invertebrates from disease is shown. In one embodiment, dsRNA or antisense RNA to a nucleic acid molecule of the disease-causing microorganism is prepared and delivered to the animal. In another embodiment, a nucleic acid molecule of the disease-causing microorganism is delivered to the animal. In another embodiment, the RNA or nucleic acid molecule is delivered to the animal by replicon particle. In a further embodiment, the protective molecule is delivered to the digestive tract of the animal. Protection from disease is obtained.
Claims20
A method of producing a composition for protecting shrimp from disease, comprising,
- a) identifying a target nucleic acid molecule of a microorganism causing said disease;
- b) preparing a protective molecule selected from the group consisting of:
- (i) a dsRNA molecule comprising at least one RNA molecule that is capable of hybridizing to said target nucleic acid molecule of said microorganism;
- (ii) an antisense RNA molecule comprising a sequence that is the full or partial complement of said target nucleic acid molecule of said microorganism;
- and
- c) preparing an orally acceptable composition comprising said protective molecule with feed, wherein said composition does not comprise an expression cassette or vector comprising said protective molecule, and wherein when orally administered to said shrimp said composition protects said shrimp from said disease.
A method of producing a composition for protecting shrimp from White Spot Syndrome Virus (WSSV) or Infectious Myonecrosis Virus (IMNV), comprising,
- a) identifying a target nucleic acid molecule of said WSSV or IMNV;
- b) preparing a protective molecule selected from the group consisting of:
- (i) a dsRNA molecule comprising at least one RNA molecule that is capable of hybridizing to said target nucleic acid molecule of said WSSV or IMNV;
- (ii) an antisense RNA molecule comprising a sequence that is the full or partial complement of said target nucleic acid molecule of said WSSV or IMNV;
- and
- c) preparing an orally acceptable composition comprising said protective molecule with feed, wherein does not comprise an expression cassette or vector comprising said protective molecule, and wherein when orally administered to said shrimp said composition protects said shrimp from said WSSV or IMNV.
A method of producing a composition for protecting shrimp from disease, comprising,
- a) identifying a target nucleic acid molecule of a microorganism causing said disease;
- b) preparing a protective molecule selected from the group consisting of:
- (i) a dsRNA molecule comprising at least one RNA molecule that is capable of hybridizing to said target nucleic acid molecule of said microorganism;
- (ii) an antisense RNA molecule comprising a sequence that is the full or partial complement of said target nucleic acid molecule of said microorganism;
- and
- c) preparing an orally acceptable composition for delivery to the digestive tract of said shrimp, said composition comprising said protective molecule with feed, wherein said composition does not comprise an expression cassette or vector comprising said protective molecule, and wherein when orally administered to said shrimp said composition protects said shrimp from said disease.
Dependencies are inferred from the claim text.