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US 9,650,608 B2Granted
Activating adipose-derived stem cells for transplantation
AssigneeMedivet America, LLC
InventorsShaneel Masood Khan, Francis Edward Dwulet, Andrew Gregory Breite
Life of this patent
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Abstract
A method of preparing a stem cell preparation is provided. The method includes steps of collecting adipose tissue from a mammal, contacting that adipose tissue with an enzyme preparation to digest fat and connective tissue and preserve stem cells and collecting those stem cells. In addition a kit is provided for preparing a stem cell preparation.
Claims16
A method of preparing an adipose-derived mesenchymal stem cell preparation for treating a bone, ligament, tendon or cartilage injury in an animal, comprising:
- a. collecting adipose tissue from a source animal, said adipose tissue including fat, connective tissue and mesenchymal stem cells;
- b. contacting said adipose tissue with an enzyme preparation comprising 55-65 wt % collagenase from Clostridium histolyticum and 35-45 wt % protease from Bascillus thermoproteolyticus followed by incubation with a cell extraction medium;
- c. isolating mesenchymal stem cells after step (b);
- d. incubating isolated mesenchymal stem cells from step c with a platelet rich plasma derived from the source animal to form a mixture;
- e. photoactivating the mixture from step d;
- and
- f. administering the mixture to the animal.
4 more elements
Dependencies are inferred from the claim text.