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US 9,650,600 B2Granted
Mycoplasma hyopneumoniae vaccine
AssigneeZoetis Services LLC
InventorsJeffrey E. Galvin, Gregory P. Nitzel, John Keith Garrett, James R. Kulawik, II, Tracy L. Ricker, Megan Marie Smutzer
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Abstract
This invention provides an immunogenic composition including a soluble portion of a Mycoplasma hyopneumoniae (M.hyo) whole cell preparation, wherein the soluble portion of the M.hyo preparation is substantially free of both (i) IgG and (ii) immunocomplexes comprised of antigen bound to immunoglobulin.
Claims13
A method for preparing an immunogenic composition including a soluble portion of a Mycoplasma hyopneumoniae (M.hyo) whole cell preparation, the method comprising:
- i) culturing M.hyo in a suitable media over periods ranging from 18-144 hours;
- ii) subsequently inactivating the M.hyo culture;
- iii) harvesting the inactivated culture fluid, wherein the inactivated culture fluid comprises an M.hyo whole cell preparation comprising both a soluble liquid fraction and insoluble cellular material;
- iv) separating the soluble liquid fraction from the insoluble cellular material;
- and
- v) substantially removing both IgG and antigen/immunoglobulin immunocomplexes from the separated soluble liquid fraction to form a soluble portion of an M.hyo whole cell preparation.
3 more elements
Dependencies are inferred from the claim text.