Recombinant mumps virus vaccine
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorBiao He
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Abstract
The present invention provides the complete genomic sequence of the epidemic mumps virus (MuV) strain MuVIowa/us/06. Further, a reverse genetics system was constructed and used to rescue recombinant viral constructs that are attenuated compared to MuVIowa/us/06 and JL vaccine viruses. Such constructs include viral constructs lacking the open reading frame (ORF) of the SH gene (rMuVΔSH) and/or incapable of expressing the V protein (rMuVΔV).
Claims24
An isolated nucleotide sequence comprising a cDNA sequence encoding the full length RNA genome of a mumps virus, wherein the cDNA sequence encoding the full length RNA genome of the mumps virus comprises one or more mutations to the V/I/P gene abrogating expression of a V protein product, wherein the mumps virus is an infectious mumps virus, wherein the one or more mutations to the V/I/P gene abrogating expression of the V protein comprise replacing the nucleotide sequence GGGGGG with the nucleotide sequence GAGGAGGG at the editing site in the P/V gene, wherein only a transcript encoding the P protein is generated from the P/V transcript.
Dependencies are inferred from the claim text.