Back to results
US 9,645,153 B2Granted
Labeling reagents and methods of their use
AssigneeLife Technologies Corporation
InventorsEvan Antoulinakis, Kyle Gee, Aleksey Rukavishnikov
Life of this patent
Estimating the term…
Abstract
The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.
Claims17
A method of making a compound of Formula I comprising:
- contacting a carrier molecule or a solid support comprising a nucleophile with a compound of Formula IA or a tautomer or salt thereof:
- wherein,
- L is a linker;
- R1 is chlorine;
- R2 is chlorine;
- R3 is COO−, SO3−, substituted azenyl, PEG, phosphate or bisphosphonate;
- and
- Ra is a reporter molecule;
- forming a compound of Formula I or a tautomer or salt thereof:
- wherein
- L is the linker;
- Ra is the reporter molecule;
- and
- Rb is the carrier molecule or solid support comprising a nucleophile (X).
11 more elements
A method of labeling a carrier molecule or solid support comprising:
- contacting the carrier molecule or solid support with a compound of Formula IA or a tautomer or salt thereof:
- wherein,
- L is a linker;
- R1 is chlorine;
- R2 is chlorine;
- R3 is COO−, SO3−, substituted azenyl, PEG, phosphate or bisphosphonate;
- and
- Ra is a reporter molecule;
- and
- the carrier molecule or solid support comprises a nucleophile;
- and
- forming a labeled carrier molecule or solid support.
9 more elements
Dependencies are inferred from the claim text.