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US 9,637,769 B2Granted
Reduction of formation of amidated amino acids in cell lines for protein expression
AssigneeLek Pharmaceuticals D.D.
InventorsMihaela {hacek over (S)}kulj, Dominik Gaser
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Abstract
The present invention is related to a method to reduce peptide amidation activity in a given cell line, cell lines with reduced peptide amidation activity, and uses thereof.
Claims24
A cell transformed to express a heterologous protein having reduced peptide amidation, wherein the expression of a gene or translation of a gene encoding an enzyme catalyzing peptide a-amidation in the cell is inhibited or reduced.
A method for the recombinant production of a heterologous protein having reduced peptide amidation, wherein said method comprises:
- a) transforming a cell express a heterologous protein, wherein the expression of a gene or translation of a gene encoding an enzyme catalyzing peptide a-amidation in the cell is inhibited or reduced, and
- b) culturing said cell under conditions to express said heterologous protein.
Dependencies are inferred from the claim text.