Methods of producing 7-carbon chemicals via carbon chain elongation associated with cyclohexane carboxylate synthesis

A method for biosynthesizing a product selected from the group consisting of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, said method comprising:

• (i) contacting 2-oxoglutarate with enzymes comprising a homocitrate synthase classified under EC 2.3.3.14 or EC 2.3.3.13, a homocitrate dehydratase and a homoaconitate hydratase classified under EC 4.2.1.114, EC 4.2.1.36 or EC 4.2.1.33 an iso(homo)ncitrate dehydrogenase classified under EC 1.1.1.85, EC 1.1.1.87, or EC 1.1.1.286, an indolepyruvate decarboxylase classified under EC 4.1.1.43 or EC 4.1.1.74, a glutarate semialdehyde dehydrogenase classified under EC 1.2.1.20, 1.2.1.16, or EC 1.2.1.79, and a glutarate:CoA ligase classified under EC 6.2.1.6 to produce glutaryl-CoA, or
• contacting acetyl-CoA with enzymes comprising a β-ketothiolase classified under EC 2.3.1.9, an acetyl-CoA carboxylase classified under EC 6.4.1.2, an acetoacetyl-CoA synthase classified under EC 2.3.1.194, a 3-hydroxyacyl-CoA dehydrogenase classified under EC 1.1.1.-, an enoyl-CoA reductase classified under 4.2.1.-, a glutaconyl-CoA decarboxylase classified under EC 4.1.1.70, and an enoyl-[acp] reductase classified under EC 1.3.1.9 or a trans-2-enoyl-CoA reductase classified under EC 1.3.1.44 or a glutaryl-CoA dehydrogenase to produce glutaryl-CoA;
• (ii) contacting said glutaryl-CoA with enzymes comprising a β-ketothiolase classified under EC 2.3.1.- or a β-ketoacyl-[acp] synthase classified under EC 2.3.1.41, EC 2.3.1.179, EC 2.3.1.180, a 3-hydroxyadipyl-CoA dehydrogenase classified under EC 1.1.1.157, an enoyl-CoA hydratase, and an enoyl-[acp] reductase classified under EC 1.3.1.9 or a trans-2-enoyl-CoA reductase classified under EC 1.3.1.44 to produce pimeloyl-CoA, or
• contacting said glutaryl-CoA with enzymes comprising a β-ketothiolase classified under EC 2.3.1.- or a β-ketoacyl-[acp] synthase classified under EC 2.3.1.41, EC 2.3.1.179, EC 2.3.1.180, a 6-oxo-cyclohex-1-ene-carbonyl-CoA hydrolase classified under EC 3.7.1.-, a 6-hydroxycyclohex-1-ene-1-carboxyl-CoA dehydrogenase classified under EC 1.1.1.-, a cyclohexa-1,5-dienecarbonyl-CoA hydratase classified under EC 4.2.1.100, a cyclohex-1-ene-1-carboxyl-CoA dehydrogenase classified under EC 1.3.8.-, a cyclohex-1-ene-1-carboxyl-CoA hydratase classified under EC 4.2.1-, a 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase classified under EC 1.1.1.-, and a 2-ketocyclohexanecarboxyl-CoA hydrolase classified under EC 4.1.3.36 to produce pimeloyl-CoA;
• (iii) contacting said pimeloyl-CoA with enzymes comprising a thioesterase classified under EC 3.1.2., an aldehyde dehydrogenase classified under EC 1.2.1.3, a 6-oxohexanoate dehydrogenase classified under EC 1.2.1.63, a 7-oxoheptanoate dehydrogenase classified under EC 1.2.1.-, a glutaconate-CoA transferase classified under EC 2.8.3.12, or a reversible CoA-ligase classified under EC 6.2.1.5 to produce pimelic acid or 7-hydroxyheptanoate, and/or contacting said pimeloyl-CoA with enzymes comprising an ω-transaminase classified under EC 2.6.1.- or a deacetylase classified under 3.5.1.62 to produce 7-aminoheptanoate or heptamethylenediamine;
• and
• (iv) optionally, contacting said 7-hydroxyheptanoate from step (iii) with an alcohol dehydrogenase classified under EC 1.1.1.- to produce 1,7-heptanediol;
• wherein at least one of said enzymes is a heterologous enzyme.