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US 9,637,758 B2Granted
Modified Sendai virus vaccine and imaging vector
AssigneeSt. Jude Children's Research Hospital
InventorsJulia Lea Hurwitz, Toru Takimoto, Charles John Russell, Allen Portner, Karen Slobod
Life of this patent
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Abstract
The present invention relates to a Sendai virus or recombinant Sendai virus vector. In particular the present invention provides methods, vectors, formulations, compositions, and kits for a modified Enders strain Sendai viral vector. An immunogenic vector can be used in any in vitro or in vivo system. Moreover, some embodiments include vectors for imaging virus growth, location and transmission.
Claims15
An immunogenic recombinant mutant Enders Sendai viral vector comprising
- a) a modified Enders Sendai virus L gene that encodes a modified L protein containing one or more amino acid mutations of the wild type L protein selected from the group consisting of wild type amino acid S to mutant amino acid G at position 155, wild type amino acid R to mutant amino acid K at position 258, wild type amino acid G to mutant amino acid E at position 466, wild type amino acid G to mutant amino acid E at position 482, wild type amino acid S to mutant amino acid R at position 581, wild type amino acid Q to mutant amino acid R at position 717, wild type amino acid T to mutant amino acid I at position 800, and wild type amino acid R to mutant amino acid K at position 852, and
- b) one or more heterologous genes that comprise a gene encoding an antigen.
Dependencies are inferred from the claim text.