Methods of producing and using regulatory B-cells
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsCharles Emeka Egwuagu, Ren-Xi Wang, Cheng-rong Yu
Life of this patent
Estimating the term…
Abstract
The invention is directed to a method of preparing B-cells that produce interleukin-10 (IL-10), or IL-10 per se, which comprises contacting one or more B-cells ex vivo with an isolated interleukin-35 (IL-35) protein, and culturing the one or more B-cells under conditions to provide one or more B-cells that produce IL-10. The invention also is directed to a method of suppressing the proliferation of lymphocytes in vitro or in vivo by contacting lymphocytes with an isolated IL-35 protein. The invention further is directed to a method of suppressing autoimmunity in a mammal by administering to the mammal an IL-35 protein or IL-10-producing B-cells.
Claims24
A method of preparing B-cells that produce interleukin-10 (IL-10), which method comprises contacting one or more B-cells ex vivo with an isolated interleukin-35 (IL-35) protein, and culturing the one or more B-cells under conditions to provide one or more B-cells that produce IL-10.
A method of producing IL-10, which method comprises contacting one or more B-cells ex vivo with an isolated interleukin-35 (IL-35) protein, and culturing the one or more B-cells under conditions to provide one or more B-cells that produce IL-10.
Dependencies are inferred from the claim text.