Metabolic engineering of plants for increased homogentisate and tocochromanol production
AssigneeThe Curators of the University of Missouri
InventorsGary Stacey, Minviluz G. Stacey
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Abstract
The invention provides methods and compositions for modifying tocochromanol biosynthesis in plants. The present invention thus surprisingly and beneficially provides for increasing desired tocochromanol compounds in plants and enhancing the nutritional quality of human food and animal feed, without associate deleterious plant phenotypes.
Claims12
A plant comprising a mutated Glyma12g20220 allele, a representative sample of seed comprising said mutated allele having been deposited under ATCC Accession No. PTA-12919.
A method of increasing production of homogentisic acid (HGA) or a metabolic product derived therefrom in a plant comprising down-regulating homogentisic acid dioxygenase (HGO) in the plant relative to a wild-type plant, wherein the plant comprises a mutated Glyma12g20220 allele, a representative sample of seed comprising said mutated allele having been deposited under ATCC Accession No. PTA-12919, and wherein said down-regulating comprises introgressing into the plant the mutated Glyma12g20220 allele.
A method of increasing tolerance to an herbicidal inhibitor of ρ-hydroxyphenylpyruvate dioxygenase (HPPD) in a plant comprising down-regulating homogentisic acid dioxygenase (HGO) in the plant relative to a wild-type plant, wherein the plant comprises increased tolerance to an herbicidal inhibitor of ρ-hydroxyphenylpyruvate dioxygenase (HPPD) and produces seeds comprising normal germination relative to a wild type plant, wherein said down-regulating comprises introgressing into the plant a mutated Glyma12g20220 allele;
- obtaining a plurality of plants with down-regulated HGO;
- and
- selecting from the plurality of plants a plant exhibiting an increased level of herbicide tolerance relative to the wild-type plant.
Dependencies are inferred from the claim text.