Method for retrovirus removal
AssigneeEMD Millipore Corporation
InventorsOnur Y. Kas, Mikhail Kozlov, Wilson Moya, Philip Goddard, Sherry Ashby Leon, Jibin Hao, Gabriel Tkacik
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Abstract
A method for removing retroviruses from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention is disclosed. Retroviruses are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a retrovirus LRV greater than about 6, and the nanofiber(s) has a diameter from about 10 nm to about 100 nm. The filtration medium can be in the form of a fibrous electrospun polymeric nanofiber liquid filtration medium mat.
Claims20
A method of removing retroviruses from a liquid sample comprising the steps of:
- a) providing a liquid sample containing retroviruses,
- b) providing a filtration medium comprising a porous nanofiber mat made by electrospinning a polymer, wherein the porous nanofiber mat has a retrovirus Log Reduction Value (LRV) greater than about 6, a porosity from about 80% to about 95%, and a liquid permeability greater than about 100 LMH/psi, and wherein the nanofiber mat has a fiber diameter effective for retention of retroviruses by size-based exclusion, and
- c) passing the liquid sample containing the retroviruses through the filtration medium such that the retroviruses are retained by the filtration medium.
Dependencies are inferred from the claim text.