Method for the urinary detection of bladder cancer
AssigneeArray Genomics
InventorsOlivier Cussenot, François Lozach, Ian Jones, Neil Metters
Life of this patent
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Abstract
The invention relates to the diagnosis of bladder cancer and more specifically to the detection in urine samples of bladder carcinomas of the transitional type. The detection method according to the present invention enables, through the utilization of a DNA chip designed for this purpose, to determine the grade of the detected tumors.
Claims17
A DNA microarray for the detection of bladder cancer comprising, fixed on its surface, deposits of reference DNAs, said reference DNAs consisting of DNA sequences from each of the following loci of the human chromosomes:
- 3q, 8q22qter, 9p, 9q, 18q12, 1 q22-q24, 11q13, 13q, 7q, 8p, 11p, 14q22-qter, 17p and 22, wherein the DNA sequences are at least 30 bases in length.
A method for analyzing a urine sample taken from a patient, comprising:
- (i) extracting DNA contained in said urine sample taken from said patient;
- (ii) fragmenting the DNA extracted in step (i);
- (iii) marking the obtained DNA fragments uniformly with a marking agent so as to form a pool of marked DNA;
- (iv) forming at least one aliquot from the pool of marked DNA and bringing each aliquot into contact with a set of reference DNAs, said contact being carried out under conditions enabling specific hybridization of the marked DNA fragments with said reference DNAs;
- said reference DNAs comprising DNA sequences from the following loci present in the human chromosomes:
- 3q, 8q22qter, 9p, 9q, 18q12, 1 q22-q24, 11q13, 13q, 7q, 8p, 11p, 14q22-qter, 17p and 22, wherein the reference DNAs are at least 30 bases in length;
- (v) eliminating the marked DNA fragments which are not specifically hybridized to the reference DNAs;
- and
- (vi) determining the intensity of the signal produced by the marked fragments hybridized to each of the selected reference DNAs.
Dependencies are inferred from the claim text.