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AssigneeVirginia Tech Intellectual Properties, Inc.
InventorsXiang-Jin Meng, Nathan M. Beach, Sheela Ramamoorthy
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The present invention provides a novel chimeric porcine circovirus infectious DNA clone and live attenuated chimeric virus with the PCV2, preferably of subtype PCV2b, capsid gene integrated into a non-pathogenic PCV1 virus genome. In a particular embodiment, the PCV2 capids gene is of subtype PCV2b, the predominant subtype circulating in pigs worldwide. The attenuated chimeric virus, designated PCV1-2b, effectively protects pigs from PCV2b challenges, and can be used as a live vaccine, as well as an inactivated (killed) vaccine, that provides protection and cross protection against PCV2b and PCV2a subtypes infection. The live attenuated vaccine of the present invention is also effective protecting pigs from porcine circovirus-associated disease (PCVAD).
A method of immunizing a pig against PCV2 viral infection, comprising administering to the pig an immunologically effective amount of a viral vaccine comprising a physiologically acceptable carrier and an immunogenic amount of a chimeric PCV1-2b comprising a nucleic acid molecule encoding a chimeric, nonpathogenic PCV1-2b derived from the genomic sequence of PCV1 wherein the open reading frame 2 (ORF2) of a wild-type PCV2b strain having the genomic sequence of SEQ ID NO:
A method of protecting a pig against porcine circovirus-associated disease (PCVAD), comprising administering to the pig an immunologically effective amount of a viral vaccine comprising a physiologically acceptable carrier and an immunogenic amount of a chimeric PCV1-2b comprising a nucleic acid molecule encoding a chimeric, nonpathogenic PCV1-2b derived from the genomic sequence of PCV1 wherein the open reading frame 2 (ORF2) of a wild-type PCV2b strain having the genomic sequence of SEQ ID NO:
Dependencies are inferred from the claim text.