Liquid viral formulations
AssigneeOncolytics Biotech Inc.
InventorsMatthew C. Coffey, Sarah Serl, Leo Pavliv
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Abstract
Provided herein are liquid viral formulations useful for the stabilization and storage of viruses and methods of preparing these formulations. The liquid viral formulations described herein include a virus (e.g., a purified virus) and a non-viral composition including excipients and a liquid carrier. The formulations can be used, for example, to retain the infectivity or immunogenicity of viruses during periods of storage.
Claims32
A viral formulation, comprising:
- (a) a purified virus;
- and
- (b) a non-viral composition comprising:
- (i) sucrose in a concentration of less than 4.5% based on the weight of the non-viral composition;
- (ii) Mg2+;
- (iii) a non-ionic surfactant;
- and
- (iv) a liquid carrier,
- wherein the non-viral composition is free of non-sucrose polyols, and carboxylates;
- wherein monovalent cationic salts are not present in the non-viral composition as excipients contributing to the stability of the virus;
- and
- wherein the non-viral composition is provided in an amount effective to stabilize the purified virus.
A viral formulation, consisting essentially of:
- (a) a purified virus;
- and
- (b) a non-viral composition comprising:
- (i) sucrose in a concentration of less than 4.5% based on the weight of the non-viral composition;
- (ii) Mg2+;
- (iii) a non-ionic surfactant;
- and
- (iv) a liquid carrier,
- wherein monovalent cationic salts are not present in the non-viral composition as excipients contributing to the stability of the virus and wherein the non-viral composition is provided in an amount effective to stabilize the purified virus.
A method of making a viral formulation, comprising the steps of:
- (a) providing a virus;
- and
- (b) combining the virus with a non-viral composition comprising:
- (i) sucrose in a concentration of less than 4.5% based on the weight of the non-viral composition;
- (ii) Mg2+;
- (iii) a non-ionic surfactant;
- and
- (iv) a liquid carrier,
- wherein the non-viral composition is free of non-sucrose polyols, and carboxylates;
- wherein monovalent cationic salts are not present in the non-viral composition as excipients contributing to the stability of the virus;
- and
- wherein the non-viral composition is provided in an amount effective to stabilize the virus,
- to form a viral formulation.
A method of making a viral formulation, comprising the steps of:
- (a) providing a virus;
- and
- (b) combining the virus with a non-viral composition consisting essentially of:
- (i) sucrose in a concentration of less than 4.5% based on the weight of the non-viral composition;
- (ii) Mg2+;
- (iii) a non-ionic surfactant;
- and
- (iv) a liquid carrier,
- wherein monovalent cationic salts are not present in the non-viral composition as excipients contributing to the stability of the virus and wherein the non-viral composition is provided in an amount effective to stabilize the virus,
- to form a viral formulation.
Dependencies are inferred from the claim text.