Engineered tyrosine ammonia lyase
AssigneeCodexis, Inc.
InventorsGjalt W. Huisman, Nicholas J. Agard, David Elgart, Xiyun Zhang
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Abstract
The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.
Claims12
A recombinant tyrosine ammonia lyase comprising an amino acid sequence comprising at least 90%, sequence identity to SEQ ID NO:
- 14, wherein said recombinant tyrosine ammonia lyase comprises a mutation at position 107 and at least one mutation selected from the group consisting of position 18, 47, 54, 59, 64, 73, 77, 88, 91, 93, 95, 97, 108, 214, 219, 222, 253, 304, 307, 315, 364, 367, 389, 394, 396, 400, 401, 423, 447, 453, 462, 490, 500, 503, 521, 550, 554, 564, and 56 of SEQ ID NO:
- 14 and wherein said recombinant tyrosine ammonia lyase is thermostable.
Dependencies are inferred from the claim text.