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AssigneeResearch Corporation Technologies, Inc.
InventorsKurt R. Gehlsen, Timothy David Jones, Francis Joseph Carr, Arron Hearn
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The present application relates to modified T cell epitopes derived from fungal ribotoxins, including a-sarcin, clavin, gigantin, mitogillin, and restrictocin, as well as modified ribotoxin molecules comprising one or more of the modified epitopes. The modified ribotoxin molecules inhibit protein synthesis, like the wild type ribotoxins, but exhibit reduced immunogenicity as compared to the corresponding wild type ribotoxin. Another aspect relates to a fusion protein which comprises a modified ribotoxin fused or conjugated or otherwise linked to a targeting molecule that is effective for binding a target of interest. Another aspect relates to the use of the modified ribotoxin or fusion protein for treating or managing a disease or condition.
A modified sarcin polypeptide comprising at least one mutation as compared to a wild type α-sarcin polypeptide having the amino acid sequence of SEQ ID NO:1, wherein the at least one mutation is at one or more of amino acids D9, Q10, P13, T15, N16, or Y18 of the wild type α-sarcin polypeptide and is within a first T cell epitope of the wild type α-sarcin polypeptide and/or is at one or more of amino acids I134, K139, E140, or Q142 of the wild type α-sarcin polypeptide and is within a second T cell epitope of the wild type α-sarcin polypeptide, wherein the first T cell epitope consists of the amino acid sequence of SEQ ID NO:6, and the second T cell epitope consists of the amino acid sequence of SEQ ID NO:4, and wherein the modified sarcin polypeptide inhibits protein synthesis and elicits a reduced T cell response as compared to the wild type α-sarcin polypeptide, wherein the inhibition of protein synthesis is measured using an in vitro transcription and translation assay (IVTT).
A modified sarcin polypeptide comprising at least one mutation as compared to a wild type α-sarcin polypeptide having the amino acid sequence of SEQ ID NO:1, wherein the at least one mutation is within a first T cell epitope and/or a second T cell epitope of the wild type α-sarcin polypeptide, wherein the at least one mutation within the first T cell epitope is one or more of D9A, D9T, Q10K, Q10R, Q10A, P13I, T15G, T15Q, T15H, N16R, N16K, N16A, Y18H, Y18K, or Y18R and wherein the at least one mutation within the second T cell epitope is one or more of I134A, K139D, K139E, K139G, K139Q, K139H, K139N, E140D, Q142D, Q142N, Q142T, Q142E, Q142R, or Q142G, and wherein the first T cell epitope consists of the amino acid sequence of SEQ ID NO:6 and the second T cell epitope consists of the amino acid sequence of SEQ ID NO:4.
Dependencies are inferred from the claim text.