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US 9,599,604 B2Granted
Flow chambers, methods of using the flow chamber, and methods of making the flow chamber
AssigneeUniversity of Florida Research Foundation, Inc.
InventorPeter S. McFetridge
Life of this patent
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Abstract
In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to a device (or “flow chamber”), methods of making a device, methods of using a device, and the like.
Claims20
A device comprising:
- a first plate and a second plate defining a flow chamber between the plates, the first plate configured to hold an independent substrate comprising a sample and comprising a securing structure for securing the substrate within the flow chamber, and the second plate comprising an integrated, recessed, optically transparent viewing window, a top surface of the viewing window being recessed below a top surface of the second plate and defining a recessed area in the second plate, and a bottom surface of the viewing window forming a portion of the flow chamber adjacent the sample to allow viewing of the sample through the viewing window;
- a first inlet port at a first end of the device that is configured to flow a first fluid;
- a flow channel within the flow chamber defined in part by the second plate and in part by the first plate, the substrate, or both, wherein the flow channel is in fluidic communication with the first inlet port and is configured so the first fluid contacts a side of the sample adjacent the viewing window and the sample is viewed through the optically transparent viewing window in real time as the sample is exposed to the first fluid;
- and an exit port that is in fluidic communication with the flow channel, wherein the flow channel is configured so that the first fluid flows from the first inlet port side to the exit port side of the flow channel.
1 more element
A flow chamber assembly comprising:
- a base plate configured to hold a substrate comprising a sample, the base plate comprising a securing structure for securing the substrate within the flow chamber;
- and
- a flow chamber plate configured to fit over the base plate, such that a first flow channel is defined by the base plate, substrate, and flow chamber plate, the flow chamber plate comprising:
- a top surface;
- a bottom surface configured to secure to the base plate;
- a first inlet port at a first end of the flow chamber plate that is in fluidic communication with the flow channel and is configured to flow a first fluid such that the first fluid contacts a first surface of the substrate facing the flow chamber plate;
- an exit port at a second end of the device that is in fluidic communication with the flow channel and is also configured to flow a first fluid;
- and
- an optically transparent viewing window comprising a transparent material having a lower surface defining a portion of the flow channel above the substrate and having an upper surface recessed below the top surface of the flow chamber plate, wherein the optically transparent viewing window is configured to allow viewing of the substrate through the transparent material,
- wherein the flow chamber plate is configured so that the first fluid flows from the first inlet port side, through the flow channel where it contacts the first surface of the substrate, to the exit port side of the flow channel, such that the substrate is viewed in real time as the substrate is exposed to the first fluid.
7 more elements
Dependencies are inferred from the claim text.