Methods and compositions for processing biomass with elevated levels of starch
AssigneeAgrivida, Inc.
InventorsPhilip A. Lessard, Michael Lanahan, Vladimir Samoylov, Oleg Bougri, Jonas Emery, R. Michael Raab, Dongcheng Zhang
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Abstract
Genetically engineered plants having altered levels of one or more starch regulation enzymes and a polysaccharide degrading enzyme are provided. Methods of genetically engineering plants to express products altering expression of one or more starch regulation enzymes and polysaccharide degrading enzymes, and genetic constructs are provided. Methods of agricultural processing and animal feed using the genetically engineered plants are described.
Claims29
A genetically engineered plant comprising:
- a first isolated nucleic acid comprising a sequence of SEQ ID NO:
- 50 that encodes a ribonucleic acid product that inactivates or inhibits expression of at least one gene encoding a protein involved in mobilization of starch in a plant, and a second isolated nucleic acid that encodes an endo-1,4-beta-xylanase from Thermomyces lanuginosus, a cellobiohydrolase B and an endo-beta 1,4-endoglucanase from Nasutitermes takasagoensis;
- wherein the second isolated nucleic acid comprises a sequence of:
- SEQ ID NO:
- 86, SEQ ID NO:
- 87, and SEQ ID NO:
- 90.
A genetic construct comprising:
- a first isolated nucleic acid comprising a sequence of SEQ ID NO:
- 50 that encodes a ribonucleic acid product that inactivates or inhibits expression of at least one gene encoding a protein involved in mobilization of starch in a plant, and a second isolated nucleic acid that encodes an endo-1,4-beta-xylanase from Thermomyces lanuginosus, a cellobiohydrolase B and an endo-beta 1,4-endoglucanase from Nasutitermes takasagoensis;
- wherein the second isolated nucleic acid comprises a sequence of:
- SEQ ID NO:
- 86, SEQ ID NO:
- 87, and SEQ ID NO:
- 90.
A method of agricultural processing or preparing animal feed comprising pretreating a genetically engineered plant with a chemical formulation to form a mixture, wherein the genetically engineered plant comprises:
- a first isolated nucleic acid comprising a sequence of SEQ ID NO:
- 50 that encodes a ribonucleic acid product that inactivates or inhibits expression of at least one gene encoding a protein involved in mobilization of starch in a plant, and a second isolated nucleic acid that encodes an endo-1,4-beta-xylanase from Thermomyces lanuginosus, a cellobiohydrolase B and an endo-beta 1,4-endoglucanase from Nasutitermes takasagoensis;
- wherein the second isolated nucleic acid comprises a sequence of:
- SEQ ID NO:
- 86, SEQ ID NO:
- 87, and SEQ ID NO:
- 90.
A method of preparing animal feed comprising at least one procedure selected from the group consisting of:
- drying a genetically engineered plant, pelletizing a genetically engineered plant into feed pellets, ensiling a genetically engineered plant to make silage, and combining the genetically engineered plant with distillers grains or with a source of edible fiber, wherein the genetically engineered plant comprises a first isolated nucleic acid comprising a sequence of SEQ ID NO:
- 50 that encodes a ribonucleic acid product that inactivates or inhibits expression of at least one gene encoding a protein involved in mobilization of starch in a plant, and a second isolated nucleic acid that encodes an endo-1,4-beta-xylanase from Thermomyces lanuginosus, a cellobiohydrolase B and an endo-beta 1,4-endoglucanase from Nasutitermes takasagoensis;
- wherein the second isolated nucleic acid comprises a sequence of:
- SEQ ID NO:
- 86, SEQ ID NO:
- 87, and SEQ ID NO:
- 90.
A method of agricultural processing comprising feeding an animal with an animal feed comprising a genetically engineered plant to promote animal growth, wherein prior to the step of feeding the animal feed is prepared by at least one procedure selected from the group consisting of:
- drying a genetically engineered plant, pelletizing a genetically engineered plant into feed pellets, ensiling a genetically engineered plant to make silage, and combining the genetically engineered plant with distillers grains or with a source of edible fiber, and the genetically engineered plant comprises a first isolated nucleic acid comprising a sequence of SEQ ID NO:
- 50 that encodes a ribonucleic acid product that inactivates or inhibits expression of at least one gene encoding a protein involved in mobilization of starch in a plant, and a second isolated nucleic acid that encodes an endo-1,4-beta-xylanase from Thermomyces lanuginosus, a cellobiohydrolase B and an endo-beta 1,4-endoglucanase from Nasutitermes takasagoensis;
- wherein the second isolated nucleic acid comprises a sequence of:
- SEQ ID NO:
- 86, SEQ ID NO:
- 87, and SEQ ID NO:
- 90.
Dependencies are inferred from the claim text.