Production of dentin, cementum and enamel by cells
AssigneeThe Trustees of Columbia University in the City of New York
InventorsJeremy J. Mao, Mo Chen
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Abstract
Described is a method of forming a mineralized material by co-culturing epithelial cell, such as ameloblast, and mesenchymal cell, such as osteoblast or odontoblast, in a mineral-stimulating medium. Also described is a matrix seeded with epithelial cells and mesenchymal cells and infused with a mineral-stimulating medium capable of forming a mineralized material in the matrix. Methods of manufacturing such compositions and methods of treating mineralization-related conditions are also described.
Claims24
A method of forming a mineralized composition comprising:
- forming a mineralization composition comprising combining
- (i) ameloblasts;
- (ii) osteoblasts or odontoblasts;
- and
- (iii) an osteogenic medium comprising ascorbic acid and glycerophosphate;
- and
- adding amelogenin into the osteogenic medium in an amount sufficient to increase nuclear translocalization of β-catenin, increase activation of β-catenin, or increase accumulation of non-phosphorylated β-catenin;
- culturing the mineralization composition under conditions suitable to induce production of a mineralized material;
- and
- forming a mineralized composition comprising enamel, dentin, or cementum.
An engineered tissue composition comprising:
- (a) ameloblasts;
- (b) osteoblasts or odontoblasts;
- (c) an osteogenic medium comprising ascorbic acid, glycerophosphate, dexamethasone, and
- (d) adding amelogenin into the osteogenic medium in an amount sufficient to increase nuclear translocalization of β-catenin, increase activation of β-catenin, or increase accumulation of non-phosphorylated β-catenin;
- and
- (e) a matrix material;
- wherein,
- the ameloblasts and the osteoblasts or odontoblasts are present in the matrix material;
- the osteogenic medium is infused in the matrix material;
- and
- the ameloblasts and the osteoblasts or odontoblasts are fluidly connected through the osteogenic medium.
A method of forming a mineralized composition comprising:
- differentiating a first plurality of progenitor cells to form ameloblasts;
- differentiating a second plurality of progenitor cells to form osteoblasts or odontoblasts;
- forming a mineralization composition comprising combining
- (i) the ameloblasts;
- (ii) the osteoblasts or odontoblasts;
- and
- (iii) an osteogenic medium comprising ascorbic acid and glycerophosphate;
- adding amelogenin in an amount sufficient to increase nuclear translocalization of β-catenin, increase activation of β-catenin, or increase accumulation of non-phosphorylated β-catenin;
- culturing the mineralization composition under conditions suitable to induce production of a mineralized material;
- and
- forming a mineralized composition comprising enamel, dentin, or cementum.
A method of forming a mineralized composition comprising:
- forming a first mineralization composition comprising ameloblasts in a first osteogenic medium comprising ascorbic acid and glycerophosphate;
- forming a second mineralization composition comprising osteoblasts or odontoblasts in a second osteogenic medium comprising ascorbic acid and glycerophosphate;
- and
- adding amelogenin into the first osteogenic medium and the second osteogenic medium in an amount sufficient to increase nuclear translocalization of β-catenin, increase activation of β-catenin, or increase accumulation of non-phosphorylated β-catenin;
- culturing the first mineralization composition and the second mineralization composition under conditions suitable to induce production of a mineralized material;
- introducing the first mineralization composition and the second mineralization composition into or onto a matrix material;
- and
- forming a mineralized composition comprising enamel, dentin, or cementum.
Dependencies are inferred from the claim text.