Protein enriched microvesicles and methods of making and using the same
AssigneeTakara Bio USA, Inc.
InventorsMichael Haugwitz, Thomas Patrick Quinn, Andrew Alan Farmer, Montserrat Morell Fernández
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Abstract
Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.
Claims14
A method of preparing a micro-vesicle containing a target protein, the method comprising:
- maintaining a cell comprising:
- (a) a membrane-associated protein comprising a first dimerization domain;
- (b) a heterologous target protein comprising a second dimerization domain;
- and
- (c) a dimerization mediator;
- under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle contains a dimerized complex of the membrane-associated protein and the heterologous target protein bound to each other by the dimerization mediator;
- and
- separating the micro-vesicle from the cell.
Dependencies are inferred from the claim text.