Back to results
US 9,593,338 B2Granted
Synthetic transcriptional control elements and methods of generating and using such elements
AssigneeThe Regents of the University of California
InventorsChang Liu, Adam P. Arkin, Lei S. Qi
Life of this patent
Estimating the term…
Abstract
Provided herein are nucleic acid constructs that contain a synthetic control element that includes a cis-regulator of translation, and an adapter translation-coupled regulator of transcription. Further provided herein are nucleic acid constructs that contain nucleic acid sequences under the control of the synthetic control elements. Also provided are compositions and methods related to the nucleic acid constructs.
Claims16
A nucleic acid construct comprising a synthetic translation-coupled regulator of transcription (TCRT) comprising in a 5′ to 3′ order:
- (a) a cis-regulator of translation that is a RNA-IN variant having a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 24-28:
- and
- (b) an adapter TCRT,
- wherein (a) and (b) are operably linked so that the transcription of sequences that are 3′ of and operably linked to the synthetic TCRT is regulated by (a).
2 more elements
Dependencies are inferred from the claim text.