Double-stranded oligonucleotides
AssigneeLife Technologies Corporation
InventorsTod Woolf, Kristin Wiederholt
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Abstract
Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions.
Claims16
A composition comprising a double-stranded oligonucleotide molecule, the molecule comprising:
- a first strand and a second strand each having a length of less than 25 nucleotides and having a duplex length of at least 10 to at least 24 nucleotides;
- wherein at least one of the first strand and the second strand contains a 5′-terminal phosphorous containing moiety;
- wherein at least one but not all of the nucleotides of the first strand comprises a 2′-O-methyl modification and at least one but not all of the nucleotides of the second strand comprises a 2′-O-methyl modification, and
- wherein said double-stranded oligonucleotide molecule is chemically synthesized.
A method for introducing a double-stranded ribonucleic acid molecule comprising a first strand and a second strand into a eukaryotic cell in vitro, the method comprising contacting the eukaryotic cell with the double-stranded ribonucleic acid molecule,
- wherein the first strand and the second strand each have a length of less than 25 nucleotides and have a duplex length of at least 10 to at least 24 nucleotides,
- wherein at least one but not all of the nucleotides of the first strand of the double-stranded ribonucleic acid molecule comprises a 2′-O-methyl modification;
- wherein at least one but not all of the nucleotides of the second strand of the double-stranded ribonucleic acid molecule comprises a 2′-O-methyl modification;
- wherein at least one of the first strand and the second strand contains a 5′-terminal phosphorus containing moiety;
- and
- wherein said double-stranded ribonucleic acid molecule is chemically synthesized;
- to result in the double-stranded nucleic acid molecule being introduced into the eukaryotic cell.
Dependencies are inferred from the claim text.